High Performance Liquid Chromatography - Chemistry LibreTexts
https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_Modules_(Analytical_Chemistry)/Instrumentation_and_Analysis/Chromatography/High_Performance_Liquid_Chromatography
WEBAug 29, 2023 · Gradient Elution vs. Isocratic Elution. If the composition of the mobile phase remains constant throughout the HPLC separation, the separation is deemed an isocratic elution. Often the only way to elute all of the compounds in the sample in a reasonable amount of time, while still maintaining peak resolution, is to change the ratio of polar to ...
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